PCR/restriction fragment length polymorphism (RFLP) typing of human and poultry Campylobacter jejuni strains


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Abstract

Aims:The PCR/RFLP typing of 156 isolates Campylobacter jejuni originating from poultry and humans was performed (101 human and 55 poultry strains).Methods and Results:On the basis of restrictive digest, six types were identified with AfaI, seven types with MboI and five types with HaeIII. With a combination of these three enzymes, 22 types were found. In human strains, the most frequently occurring types were Cj.4 (28%), Cj.1 (19%), Cj. 13 (13%) and Cj. 2 (5%). In the case of poultry strains, the most frequent types were Cj. 1 (34%), Cj. 11 (22%), C.j. 21 (16%) and Cj. 15 (11%).Conclusions:The findings support the hypothesis that poultry is a significant source but not sole source of Campylobacter sp. in relation to humans.Significance and Impact of the Study:The typing of Campylobacter sp. forms the basis for an evaluation of the current state and risk assessment of various Campylobacter sp. sources in relation to humans.

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