Optimization of a fermentation medium for the production of Penicillin G acylase from Bacillus sp.

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AimsOptimization of Penicillin G acylase (PAC) production from a novel isolate of Bacillus sp.MethodsFermentation medium for PAC production was optimized using a two-level fractional factorial design with seven components.ResultsA maximum production of 9·5 U ml−1 of PAC was obtained in an optimized medium containing (g l−1): K2HPO4, 1·0; MgSO4·7H2O, 0·1; CaCl2·2H2O, 0·1; PAA, 2·0; tryptone, 5·0; yeast extract, 3·0; and sucrose, 50·0.Significance and Impact of the StudyThe two-step medium optimization resulted in a twofold increase in PAC production. Since the strain Bacillus sp. PGS10 produces a high level of PAC, it could be a potential candidate for industrial production of PAC.

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