Identification of heterofermentative lactobacilli isolated from pig faeces by numerical analysis of total soluble cell protein patterns and RAPD-PCR


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Abstract

Aims:The study deals with a number of heterofermentative Lactobacillus strains isolated from pig faeces and their identification.Methods and Results:SDS-PAGE of total soluble cell proteins and RAPD-PCR profiles were used to identify the strains isolated from pig faeces. Protein profiles obtained with SDS-PAGE revealed that 15 strains clustered at r >= 0·78 with Lactobacillus buchneri and nine strains at r >= 0·77 with two reference strains of Lactobacillus reuteri. The identity of the strains was confirmed with RAPD-PCR.Conclusions:Numerical analysis of protein profiles and RAPD-PCR proved valuable in the differentiation of Lactobacillus spp. isolated from pig faeces.Significance:This is the first report on the association of Lact. buchneri with pig faeces.

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