Direct PCR detection of Escherichia coli O157:H7


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Abstract

AimsThis paper reports a simple, rapid approach for the detection of Shiga toxin (Stx)-producing Escherichia coli (STEC).Methods and ResultsDirect PCR (DPCR) obviates the need for the recovery of cells from the sample or DNA extraction prior to PCR. Primers specific for Stx-encoding genes stx1 and stx2 were used in DPCR for the detection of E. coli O157:H7 added to environmental water samples and milk.ConclusionsPCR reactions containing one cell yielded a DPCR product.Significance and Impact of the StudyThis should provide an improved method to assess contamination of environmental and other samples by STEC and other pathogens.

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