Investigations on anti-Aspergillus properties of bacterial products


    loading  Checking for direct PDF access through Ovid

Abstract

AimsTo investigate the anti-Aspergillus properties of bacterial products.Methods and ResultsIn the present study, 12 bacterial strains were screened for antifungal activity against Aspergilli. The culture supernatant and lysates of Pseudomonas aeruginosa, Bacillus cereus, Escherichia coli (BL21, DH5α, HB101, XL Blue), Klebsiella pneumoniae, Streptomyces thermonitrificans, Streptococcus pneumoniae, Enterobacter aerogenes, Staphylococcus aureus and Salmonella typhi were examined for antifungal activity in protein concentration ranging from 1000·0 to 7·8 μg ml−1 using microbroth dilution assay. The lysate of Salm. typhi and E. coli BL21 exhibited the maximum activity against Aspergillus fumigatus, Aspergillus flavus and Aspergillus niger. Their in vitro minimum inhibitory concentrations (MICs) were found to be 15·6–31·2 μg ml−1 by microbroth dilution and spore germination inhibition assays. In disc diffusion assay, a concentration of 3·1 μg disc−1 of Salm. typhi lysate showed significant activity against Aspergilli. Escherichia coli BL21 exhibited similar activity at 6·2 μg disc−1. The work on identification of molecule endowed with antimycotic properties is in progress.ConclusionThe products of Salm. typhi and E. coli demonstrated significant activity against Aspergillus species.Significance and Impact of the StudyThis is the first time that E. coli has been reported for anti-Aspergillus activity. It could be an important source of biologically active compounds useful for developing better new antifungal drugs/or probiotics.

    loading  Loading Related Articles