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To examine whether water activity (aw) in combination with low concentration of fungicides can be used to effectively control Aspergillus niger van Tieghem growth in cultural medium, the Sabouraud dextrose agar (SDA). The data would be used as baseline information for reducing A. niger contamination in insect artificial diets.Aspergillus niger was isolated from an insect artificial diet. Four concentration levels (i.e. 0, 1, 10 and 20 μmol) of two fungicides (i.e. amphotericin B and itraconazole) were tested against A. niger under four aw levels (i.e. 0·994, 0·961, 0·921 and 0·859) adjusted by including 0, 12·5, 25 and 38% of glycerol in the medium mixture. Aspergillus niger growth was significantly reduced at low fungicide concentration (1 μmol), and at reduced aw. The spore germination was prevented with either higher fungicide concentration (>10 μmol), or low aw in the medium (aw < 0·921). The two ecological determinants (fungicides and aw) showed a significant impact on A. niger survival in the medium (P < 0·0001). Itraconazole is more effective than amphotericin B in controlling A. niger contamination in the agar medium.Adjustment of aw (with 12·5% of glycerol) in combination with 1 μmol of itraconazole can effectively prevent A. niger growth in the SDA cultural medium.Aspergillus niger contaminations have frequently affected the quality of insects produced from mass rearing facilities. Low aw in combination with low fungicide concentration has the potential to become one of the most cost-effective management strategies to prevent A. niger contamination in insect artificial diets. The effect of fungicides and low aw in artificial diets on insect biology needs to be further examined.