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To evaluate the efficacy of electrochemically activated solution (ECASOL™) in decontaminating Bacillus anthracis Ames and Vollum 1B spores, with and without changing the source water hardness and final ECASOL™ pH.Five different ECASOL™ formulations were generated, in which the source water hardness and final ECASOL™ pH were varied, resulting in cases where significant changes in free available chlorine (FAC) and oxidative-reduction potential (ORP) were observed. B. anthracis Ames and Vollum 1B spores were suspended in the various ECASOL™ formulations for 30 min, and decontamination efficacy was determined; calcium hypochlorite [5% high-test hypochlorite (HTH)] was used as a positive control. The five different ECASOL™ formulations yielded mean FAC levels ranging from 305 to 464 ppm, and mean ORP levels ranging from +826 to +1000 mV. Exposure to all the ECASOL™ formulations and 5% HTH resulted in ≥7·0 log reductions in both B. anthracis Ames and Vollum 1B spores.The present testing demonstrated that ECASOL™ with a minimum of c. 300-ppm FAC levels and +800-mV ORP inactivated the B. anthracis spores in suspension, similar to 5% HTH.These results provide information for decontaminating B. anthracis Ames and Vollum 1B spores in suspension using ECASOL™.