Determination of G and P type diversity of group A rotaviruses in faecal samples of diarrhoeic calves in Kashmir, India


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Abstract

Aims:To determine the genetic diversity of group A rotaviruses in bovine calves in Kashmir, India.Methods and Results:Of 200 diarrhoeic faecal samples collected from calves, aged between 0 and 6 months and screened by polyacrylamide gel electrophoresis (PAGE), 31 were detected positive for group A rotaviruses. On G and P genotyping by reverse transcriptase-polymerase chain reaction (RT-PCR), G10P[11] turned out to be predominant (80·64%) combination followed by G8P[11] (7·7%). One (3·84%) sample carried mixed infection of G8 + G10P[11]. Two (7·7%) samples belonged to P[11] genotype, but their G genotype specificity could not be established. This study revealed the ambiguity in RT-PCR typing method. All the samples that turned out to be G10 by Isegawa et al. (1993; Mol Cell Probes7, 277) primers could be amplified by G3 specific primers of Gouvea et al. (1990; J Clin Microbiol32, 1338). However, on homology study of their VP7 gene sequence, the strains turned out to be G10.Conclusions:Rotavirus is prevalent in diarrhoeic calves in Kashmir, India, and G10P[11] is the predominant genotype in circulation. There is evidence of mixed infection. Even though RT-PCR method is the quick way to type the strains, there is need to generate more sequence data to improve the specificity of typing primers.Significance and Impact of the Study:Rotavirus is a significant cause of diarrhoea in calves. RT-PCR typing method needs to be supported by the sequence data, and there is need to re-evaluate the primers used for typing.

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