Circular dichroism studies of the interaction of Tat analogues substituted in the Arg52 position with TAR RNA HIV-1

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Abstract

Summary

The Tat wild-type fragment of sequence Arg49-Lys-Lys-Arg52-Arg-Gln-Arg-Arg-Arg57-NH2 (labelled as Tat1) and three analogues of this fragment with the substitution Arg52 → D-Arg52 (labelled as Tat2) or L-citrulline (Cit) (labelled as Tat3) or L-ornithine (Orn) (labelled as Tat4) were synthesized to study Tat-TAR RNA HIV-1 (27-nucleotide fragment of sequence 5′-AGAUCUGAGCCUGGAGCUCUCU-3′) interactions by circular dichroism. α-helical structure was the most readily adopted by the Tat3 analogue with Arg52 → Cit substitution. All the peptides investigated caused conformational changes in the TAR structure. The most dramatic changes were observed for the Tat2-TAR complex.

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