Behavioral tests and oxidative stress evaluation in mitochondria isolated from the brain and liver of mice treated with riparin A

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Abstract

Aims

This study aimed at evaluating the oxidative stress in mitochondria isolated from the brain and liver of mice treated with riparin A, as well as the locomotor activity and myorelaxant effect of this compound. Behavioral models of rota rod and open field tests were used for locomotor activity and myorelaxant effect evaluation.

Main methods

The animals were divided into five groups (n = 8), which were treated with: diazepam (1 mg/kg, i.p), riparin A (5, 10, and 20 mg/kg, o.r.) or vehicle (0.9% saline, o.r.). The oxidative stress evaluation was carried out in mitochondria isolated from the brain and liver of mice from five experimental groups (n = 8), which were treated with: ascorbic acid (250 mg/kg; positive control), vehicle (0.9% saline; negative control) and riparin A (5, 10 and 20 mg/kg).

Key findings

In an open field and rota rod test a significant difference in the number of crossings, in time of permanence on the swivel bar and in the number of falls in riparin A treated animals (5, 10 and 20 mg/kg) was not observed, when compared with negative control (vehicle) (p > 0.05). In comparison to the negative control, there was a reduction of lipid peroxidation levels and nitrite content in mice treated with riparin A (p < 0.05). Reduced glutathione (GSH) levels (p < 0.05), superoxide dismutase (SOD) and catalase activities increased in the brain (rip A 5 mg/kg; p < 0.05), while in the liver SOD remained unchanged (p > 0.05) and catalase activity (p < 0.05) was reduced.

Significance

Riparin A was presented as a bioactive molecule devoid of adverse effects of alteration of motor activity.

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