In-vivo occlusal caries prevention by pulsed CO2-laser and fluoride varnish treatment—A clinical pilot study

    loading  Checking for direct PDF access through Ovid


Background and Objectives:

High caries prevalence in occlusal pits and fissures warrants novel prevention methods. An 86% reduction in dental enamel smooth surface demineralization in-vivo following short-pulsed 9.6 μm-CO2-laser irradiation was recently reported. The objective of this study was to conduct a blinded 12-month-pilot clinical trial of occlusal pit and fissure caries inhibition using the same CO2-laser irradiation conditions.

Study Design/Materials and Methods:

Twenty subjects, average age 14 years, were recruited. At baseline, second molars were randomized into test and control groups, assessed by International Caries Detection & Assessment System (ICDAS-II), SOPROLIFE light-induced fluorescence evaluator in daylight and blue-fluorescence mode and DIAGNOdent. An independent investigator irradiated test molars with a CO2-laser, wavelength 9.6 μm, pulse-duration 20 μs, pulse-repetition-rate 20 Hz, beam diameter 800 μm, average fluence 4.5 ± 0.5 J/cm2, 20 laser pulses per spot. At 3-, 6- and 12-month recall teeth were assessed by ICDAS, SOPROLIFE and DIAGNOdent. All subjects received fluoride varnish applications at baseline and 6-month recall.


All subjects completed the 3-month, 19 the 6-month and 16 the 12-month recall. At all recalls average ICDAS scores had decreased for the test and increased for the control fissures (laser vs. control, 3-month: −0.10 ± 0.14, 0.30 ± 0.18, P > 0.05; 6-month: −0.26 ± 0.13, 0.47 ± 0.16, P = 0.001; 12-month: −0.31 ± 0.15, 0.75 ± 0.17, P < 0.0001; mean ± SE, unpaired t-test) being statistically significantly different at 6- and 12-month recalls.


SOPROLIFE daylight evaluation revealed at 6- and 12-months statistically significant differences in changes between baseline and recall for test and control molars, respectively (laser vs. control, 6-month: 0.22 ± 0.13, 0.17 ± 0.09, P = 0.02; 12-month: 0.28 ± 0.19, 0.25 ± 0.17, P = 0.03). For SOPROLIFE blue-fluorescence evaluation mean changes in comparison to baseline for the control and the laser treated teeth were also statistically significant for the 6- and 12-month recall.


Specific microsecond short-pulsed 9.6 μm CO2-laser irradiation markedly inhibits caries progression in pits and fissures in comparison to fluoride varnish alone over 12 months. Lasers Surg. Med. 45:302–310, 2013. © 2013 Wiley Periodicals, Inc.

Related Topics

    loading  Loading Related Articles