Direct Magnetic Resonance Imaging of Histological Tissue Samples at 3.0T

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Direct imaging of a histological slice is challenging. The vast difference in dimension between planar size and the thickness of histology slices would require an RF coil to produce a uniform RF magnetic (B1) field in a 2D plane with minimal thickness. In this work a novel RF coil designed specifically for imaging a histology slice was developed and tested. The experimental data demonstrate that the coil is highly sensitive and capable of producing a uniformB1 field distribution in a planar region of histological slides, allowing for the acquisition of high-resolutionT2 images andT2 maps from a 60-μm-thick histological sample. The image intensity andT2 distributions were directly compared with histological staining of the relative iron concentration of the same slice. This work demonstrates the feasibility of using a microimaging histological coil to image thin slices of pathologically diseased tissue to obtain a precise one-to-one comparison between stained tissue and MR images. Magn Reson Med 57:835–841, 2007. © 2007 Wiley-Liss, Inc.

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