Quantification of choline- and ethanolamine-containing metabolites in human prostate tissues using 1H HR-MAS total correlation spectroscopy


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Abstract

A fast and quantitative 2D high-resolution magic angle spinning (HR-MAS) total correlation spectroscopy (TOCSY) experiment was developed to resolve and quantify the choline- and ethanolamine-containing metabolites in human prostate tissues in ≈1 hr prior to pathologic analysis. At a 40-ms mixing time, magnetization transfer efficiency constants were empirically determined in solution and used to calculate metabolite concentrations in tissue. Phosphocholine (PC) was observed in 11/15 (73%) cancer tissues but only 6/32 (19%) benign tissues. PC was significantly higher (0.39 ± 0.40 mmol/kg vs. 0.02 ± 0.07 mmol/kg, z = 3.5), while ethanolamine (Eth) was significantly lower in cancer versus benign prostate tissues (1.0 ± 0.8 mmol/kg vs. 2.3 ± 1.9 mmol/kg, z = 3.3). Glycerophosphocholine (GPC) (0.57 ± 0.87 mmol/kg vs. 0.29 ± 0.26 mmol/kg, z = 1.2), phosphoethanolamine (PE) (4.4 ± 2.2 mmol/kg vs. 3.4 ± 2.6 mmol/kg, z = 1.4), and glycerophosphoethanolamine (GPE) (0.54 ± 0.82 mmol/kg vs. 0.15 ± 0.15 mmol/kg, z = 1.8) were higher in cancer versus benign prostate tissues. The ratios of PC/GPC (3.5 ± 4.5 vs. 0.32 ± 1.4, z = 2.6), PC/PE (0.08 ± 0.08 vs. 0.01 ± 0.03, z = 3.5), PE/Eth (16 ± 22 vs. 2.2 ± 2.0, z = 2.4), and GPE/Eth (0.41 ± 0.51 vs. 0.06 ± 0.06, z = 2.6) were also significantly higher in cancer versus benign tissues. All samples were pathologically interpretable following HR-MAS analysis; however, degradation experiments showed that PC, GPC, PE, and GPE decreased 7.7 ± 2.2%, while Cho+mI and Eth increased 18% in 1 hr at 1°C and a 2250 Hz spin rate. Magn Reson Med 60:33–40, 2008. © 2008 Wiley-Liss, Inc.

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