Oxidative DNA damage in β-thalassemic children

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Abstract

Objective

The aim of the present study was to assess DNA oxidative damage by detection of 8-hydroxydeoxyguanosine (8-OHdG), lipid peroxidation, and antioxidant status in thalassemic patients.

Materials and methods

Blood samples were collected from 94 participants (64 β-thalassemic patients and 30 healthy controls) in the range of ages 4–15 years.

Results

It was clear that markers of free oxygen radical injury in blood (i.e. 8-OHdG) were significantly elevated in β-thalassemia major (P<0.001) and β-thalassemia intermediate (P<0.001) compared with normal controls, and lipid peroxide was also significantly higher in both types of thalassemia compared with controls (P<0.001). In contrast, mean catalase levels and glutathione-S-transferase were decreased (P<0.001). There were highly significant changes in the values of 8-OHdG between the thalassemia major and thalassemia intermedia groups (P=0.000), and it was significantly correlated with serum ferritin in thalassemic children (r=0.5, P=0.006).

Conclusion

β-Thalassemic children are at higher risk for tissue injury and DNA oxidative damage due to a state of enhanced oxidative stress.

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