Development and characterization of a murine monoclonal antibody against phaeomelanin and its precursor 5-S-cysteinyldopa

    loading  Checking for direct PDF access through Ovid


This study reports our success in developing a murine monoclonal antibody (mAb) against phaeomelanin and its major precursor, 5-S-cysteinyldopa (5-S-CD). A competitive enzyme-linked Immunosorbent assay was developed and demonstrated that the new mAb, designated as 6D4 (lgG1, k), reacted with both 5-S-CD and phaeomelanin, but not with eumelanin. The concentrations required for 50% inhibition of 5-S-CD and phaeomelanin were 65 ng/ml and 95 ng/ml respectively. The minimal amount of 5-S-CD and phaeomelanin which could be detected by mAb 6D4 was approximately 5 and 6 ng/ml, respectively. The immunohistochemical assay indicated that the antigenic epitope(s) recognized by mAb 6D4 was localized In the cytoplasm of certain types of melanocytlc tumours, such as superficial spreading melanoma.

Related Topics

    loading  Loading Related Articles