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To investigate the association of the daily composition of time spent sedentary, in light physical activity and moderate-to-vigorous physical activity and time in bed (movement behaviors) with blood pressure (BP) among white- and blue-collar workers.Systolic BP (SBP), diastolic BP (DBP) and body mass index of 827 workers were objectively measured. Daily composition of movement behaviors was derived from an Actigraph placed on the thigh for 1 to 5 d using the Acti4 software (2012–2013). The composition was expressed as isometric log-ratios. The cross-sectional associations between daily movement behavior composition and BP were investigated using the Compositional Data Analysis approach. The associations were adjusted for age, sex, body mass index, lift/carry duration, medication intake, and job sector.Daily composition of time spent in movement behaviors was significantly associated with SBP (F = 2.84, P = 0.04), but not DBP (F = 0.48, P = 0.69). Specifically, time reallocation to sedentary time and light physical activity from the remaining behaviors was deleteriously associated with SBP, whereas time reallocation to time in bed and moderate-to-vigorous physical activity from the remaining behaviors was beneficially associated with SBP. However, the results were only significant for time spent sedentary (P = 0.01) and in bed (P = 0.047).Daily composition of movement behaviors is associated with SBP among workers. Spending more time sedentary compared with other behaviors was deleteriously associated with SBP, whereas spending more time in bed was beneficially associated with SBP. How time is spent in different movement behaviors throughout the day is important for BP and needs to be further investigated to be included in future clinical practice guidelines.