To investigate the role of staphylococcal enterotoxins (SEs) produced byStaphylococcus pseudintermediusin the pathogenesis of pyoderma, isolates from dogs with pyoderma and healthy dogs were analyzed. According to reverse passive latex agglutination, 14/184 isolates (7.6%) from dogs with pyoderma and 9/87 (10.3%) from healthy dogs produced SEs (SEA, SEC or SED). According to multiplex PCR, 99 isolates (53.7%) from dogs with pyoderma and 97 (90.8%) from healthy dogs possessed one or moresegenes. There was no significant difference regardingsesbetween dogs with pyoderma and healthy dogs. Therefore, SEs may not be a direct virulence factor in pyoderma.