Assessment and comparison of probiotic potential of fourLactobacillusspecies isolated from feces samples of Iranian infants

    loading  Checking for direct PDF access through Ovid


The probiotic potential ofLactobacillusspecies isolated from infant feces was investigated. For this study, the antibiotic susceptibility, tolerance in gut-related conditions, antimicrobial activity, and ability to adhere to a human colorectal adenocarcinoma cell line (Caco-2 cells) of four commonLactobacillusspecies (Lactobacillusparacasei[n =15],Lactobacillus rhamnosus[n =45],Lactobacillus gasseri[n =20] andLactobacillus fermentum[n =18]) were assessed. Most isolates that which were sensitive to imipenem, ampicillin, gentamycin, erythromycin and tetracycline were selected for other tests.L. gasseriisolates had the greatest sensitivity to gastric and intestinal fluids (<10% viability).L. fermentum(FH5, FH13 and FH18) had the highest adhesion to Caco-2 cells. The lowest antibacterial activity against pathogenic bacteria was shown byL. gasseristrains in spot tests. Furthermore, non-adjusted cell-free culture supernatants with low pH had greater antimicrobial activity, which was related to organic acid. The results showed that some isolates ofL. rhamnosusandL. fermentumare suitable for use as a probiotic.

Related Topics

    loading  Loading Related Articles