The probiotic potential ofLactobacillusspecies isolated from infant feces was investigated. For this study, the antibiotic susceptibility, tolerance in gut-related conditions, antimicrobial activity, and ability to adhere to a human colorectal adenocarcinoma cell line (Caco-2 cells) of four commonLactobacillusspecies (Lactobacillusparacasei[n =15],Lactobacillus rhamnosus[n =45],Lactobacillus gasseri[n =20] andLactobacillus fermentum[n =18]) were assessed. Most isolates that which were sensitive to imipenem, ampicillin, gentamycin, erythromycin and tetracycline were selected for other tests.L. gasseriisolates had the greatest sensitivity to gastric and intestinal fluids (<10% viability).L. fermentum(FH5, FH13 and FH18) had the highest adhesion to Caco-2 cells. The lowest antibacterial activity against pathogenic bacteria was shown byL. gasseristrains in spot tests. Furthermore, non-adjusted cell-free culture supernatants with low pH had greater antimicrobial activity, which was related to organic acid. The results showed that some isolates ofL. rhamnosusandL. fermentumare suitable for use as a probiotic.