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Although there is no distinct, absolute, and ideal method for vaginal reconstruction, intestinal transfers are preferable to the other described techniques in the main clinics. In this report, we describe the transfer of a jejunal segment for vaginal reconstruction in a 20-year-old female patient, harvesting the flap based on its vascular pedicle by means of a laparoscopic technique that obviates the use of midline laparotomy. To prevent any vascular compromise, two different vascular supply sources were provided for the flap performing additional arterial and venous microvascular anastomosis. The results have confirmed the feasibility of the technique. The functional, structural, and esthetic advantages of the jejunal flap and the lower donor site morbidity of the laparoscopy-assisted technique that were rather encouraging are described and discussed.