NF-κB modulates activation of the BMP-2 gene by trichostatin A

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Abstract

In this study, trichostatin A (TSA), a histone deacetylase inhibitor, increased the Bone morphogenetic protein-2 (BMP-2) mRNA level in a human osteoblasts line. Deletion analysis of the promoter region revealed that TSA-induced luciferase was regulated by the BMP-2 promoter spanning from −320 to −310. Electrophoresis mobility shift assay (EMSA) and Chromatin immunoprecipitation (CHIP) assay proved that this position was a nuclear factor (NF)-κB responsive element. The results above demonstrated that acetylation plays a crucial role in BMP-2 expression, and acetylation of NF-κB p65 and p50 subunits by TSA treatment may activate the BMP-2 promoter.

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