Transcriptional inhibition by a non-histone protein from low mobility group in homologous and heterologous in vitro systems

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An acidic non-histone chromosomal protein of low mobility group (LMG) with pI of 5–5.5 and molecular weight of 160 kDa (named LMG160), has been isolated from rat liver nuclei and its inhibitory effect on transcription of DNA in vitro has been detected in a heterologous prokaryotic in vitro transcription system (T7 RNA polymerase) and also confirmed in the homologous cell-free system of rat liver nuclear extract. The results indicate that in the presence of non-histone protein LMG160, in vitro RNA synthesis represses in the both systems, which can suggest a functional role for this non-histone chromosomal protein.

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