Lineage-specific activities of a multipotent mitochondrion of trypanosomatid flagellates

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Abstract

Trypanosomatids are a very diverse group composed of monoxenous and dixenous parasites belonging to the excavate class Kinetoplastea. Here we studied the respiration of five monoxenous species (Blechomonas ayalai,Herpetomonas muscarum,H. samuelpessoai,Leptomonas pyrrhocorisandSergeia podlipaevi) introduced into culture, each representing a novel yet globally distributed and/or species-rich clade, and compare them with well-studied flagellatesTrypanosoma brucei,Phytomonas serpens,Crithidia fasciculataandLeishmania tarentolae. Differences in structure and activities of respiratory chain complexes, respiration and other biochemical parameters recorded under laboratory conditions reveal their substantial diversity, likely a reflection of different host environments. Phylogenetic relationships of the analysed trypanosomatids do not correlate with their biochemical parameters, with the differences within clades by far exceeding those among clades. As theS. podlipaevicanonical respiratory chain complexes have very low activities, we believe that its mitochondrion is utilised for purposes other than oxidative phosphorylation. Hence, the single reticulated mitochondrion of diverse trypanosomatids seems to retain multipotency, with the capacity to activate its individual components based on the host environment.

Five newly isolated trypanosomatids were tested for specific mitochondrial enzymatic activities and respiration. The collected data were mapped to phylogeny to see whether there is a correlation between mitochondrial features and phylogeny or not. We concluded that trypanosomatids, in general, possess a mitochondrion that is capable of adjusting to various environments.

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