Verticillium albo-atrum culture filtrates contain numerous metabolites such as enzymes and toxins. Their purification was carried out by high phase liquid chromatography (HPLC). In this study, we set out to look for other lighter metabolites that could be identified by gas chromatography coupled to mass spectrometry (GC-MS). The analyses revealed the presence of many volatile chemical compounds, some of which are of great economic interest such as methyl paraben, propyl paraben tethane and xylene. Verticillium albo-atrum, which is a pathogen of many market-garden and fodder crops, is able to produce substances that can be used in both the agro-food and cosmetic industries.