Isozyme variation within and among populations of Microsporum speciesIsoenzym-Variationen innerhalb und zwischen Populationen von Microsporum-Arten


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Abstract

Summary.Isozyme variation among 54 isolates of Microsporum canis, 18 Microsporum cookei isolates and two Diheterospora isolates were studied using starch gel electrophoresis. Of eight enzymes examined, four were polymorphic (EST, G6P, MDH and PEP), having from two to four electrophoretic forms. Within each species, consistent and reproducible isozyme patterns of the eight enzyme systems were obtained. Phenotypic diversity (H) in M. canis was higher than in M. cookei (H = 0.459 and H = 0.408 respectively), but phenotypic differentiation of M. canis isolates from different geographical regions (Auckland, Wellington and Palmerston North, New Zealand) was low, with a proportion of total diversity (Gst) of 0.151 found among the localities. The results suggest that the isolates of M. canis from different geographical regions are closely related, supporting the theory of a common lineage.Zusammenfassung.Die Isoenzymvariationen zwischen 54 Microsporum canis-, 18 Microsporum cookei- und 2 Diheterospora-Isolaten wurden mittels Stärkegelelektrophorese untersucht. Unter 8 geprüften Enzymen erwiesen sich 4 als polymorph mit 2-4 elektrophoretischen Varianten. Innerhalb jeder Art wurden übereinstimmende und reproduzierbare Isoenzymmuster der 8 Enzymsysteme beobachtet. Die phänotypische Vielfalt war in M. canis höher als in M. cookei. Die phänotypische Differenzierung von M. canis-Isolaten aus unterschiedlichen geographischen Regionen (Auckland, Wellington und Palmerston North, Neuseeland) war gering. Die Ergebnisse lassen erkennen, daβ die M. canis-Isolate aus unterschiedlichen geographischen Regionen eng miteinander verwandt sind, was die Theorie gemeinsamer Abstammung stützt.

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