Comparative serological evaluation of 10 Blastomyces dermatitidis yeast phase lysate antigens from different sourcesVergleichende serologische Bewertung von zehn Blastomyces dermatitidis-Hefephase-Lysatantigenen verschiedener Herkünfte


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Abstract

SummaryYeast phase lysate antigens from 10 isolates of Blastomyces dermatitidis (dog, ERC-2 and T-58; T-27, polar bear; woodpile, ER-3; bat lung, 48938; human, B5929, B5895, B5896, B5931, and CAPP) from different geographical regions, in addition to a Histoplasma capsulatum (G217B) lysate preparation were compared with respect to their reactivity against serum specimens from dogs, rabbits and humans positive for blastomycosis using an indirect enzyme-linked immunosorbent assay. In addition, the lysate antigens were also assayed against histoplasmosis-positive human serum samples to study their cross-reactivity. Variable results were obtained with T-58 and T-27 exhibiting the greatest reactivity. We also noticed that the lysate did not react consistently to serum samples across species with lesser reactivity evidenced when testing dog sera. Finally, T-58 gave the highest cross-reactivity with histoplasmosis-positive sera. The study may prove valuable in the development of antigen candidates for blastomycosis serodiagnosis.ZusammenfassungHefephasen-Lysatantigene von zehn Blastomyces dermatitidis-Isolaten von Tieren und Menschen verschiedener geographischer Regionen sowie ein Histoplasma capsulatum-Antigen wurden mittels ELISA an Seren von Tieren und Menschen mit Blastomykose getestet. Variable Resultate bei größter Reaktivität wurden mit den Antigenen T-58 (Isolat vom Hund) und T-27 (Isolat vom Polarbär) beobachtet. Die Lysate reagierten nicht übereinstimmend kreuzreagierend mit Seren von Hunden. T-58 zeigte die höchste Kreuzreaktivität mit Histoplasmose-positiven Seren.

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