Disruption of Mediator rescues the stunted growth of a lignin-deficientArabidopsismutant

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Lignin is a phenylpropanoid-derived heteropolymer important for the strength and rigidity of the plant secondary cell wall1,2. Genetic disruption of lignin biosynthesis has been proposed as a means to improve forage and bioenergy crops, but frequently results in stunted growth and developmental abnormalities, the mechanisms of which are poorly understood3. Here we show that the phenotype of a lignin-deficientArabidopsismutant is dependent on the transcriptional co-regulatory complex, Mediator. Disruption of the Mediator complex subunits MED5a (also known as REF4) and MED5b (also known as RFR1) rescues the stunted growth, lignin deficiency and widespread changes in gene expression seen in the phenylpropanoid pathway mutantref8, without restoring the synthesis of guaiacyl and syringyl lignin subunits. Cell walls of rescuedmed5a/5b ref8plants instead contain a novel lignin consisting almost exclusively ofp-hydroxyphenyl lignin subunits, and moreover exhibit substantially facilitated polysaccharide saccharification. These results demonstrate that guaiacyl and syringyl lignin subunits are largely dispensable for normal growth and development, implicate Mediator in an active transcriptional process responsible for dwarfing and inhibition of lignin biosynthesis, and suggest that the transcription machinery and signalling pathways responding to cell wall defects may be important targets to include in efforts to reduce biomass recalcitrance.

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