Repeated immobilization stress reduces the gene expression of the type 1 and 2 IP3 receptors in stellate ganglia


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Abstract

Inositol 1,4,5-trisphosphate (IP3) is one of the second messengers produced by phosphoinositid hydrolysis and triggers IP3 receptor (IP3R) mediated calcium release from intracellular pools. To determine whether immobilization stress affects the gene expression and protein level of IP3R in stellate ganglia, animals were immobilized once for 2 h and/or for 7 days, 2 h daily. After decapitation, stellate ganglia were extirpated and the gene expression of IP3 receptors was evaluated. Protein levels of IP3 receptor were measured by Western blot analysis using the antibody against IP3 receptor.In the present work, we clearly show that type 1 and 2 IP3 receptors, but not the type 3 IP3 receptor, are expressed in stellate ganglia. Both types, type 1 and 2 IP3 receptors, are not significantly affected by single 2 h immobilization stress on mRNA and protein level. However, gene expression of both these types is significantly reduced by repeated immobilization stress for 7 days, 2 h daily. The IP3 receptor protein is reduced as well. Physiological relevance of our observations remains to be elucidated.

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