Expression of brain-derived neurotrophic factor in rat hippocampus following focal cerebral ischemic injury

    loading  Checking for direct PDF access through Ovid



The functional role of brain-derived neurotrophic factor (BDNF) is enhanced following cerebral ischemic injury providing neurons with an important self-protection mechanism in early stage ischemia/hypoxia.


To investigate the expression pattern of BDNF in different rat hippocampal regions following focal cerebral ischemic injury.


We performed a comparative and neurobiological study of animals in the Department of Histology and Embryology and the Central Laboratory, Hebei Medical University from March to December 2003.


Forty healthy Sprague Dawley rats were randomly divided into a cerebral ischemia group and a sham operation group, with 20 rats per group.


In the cerebral ischemia group, we occluded the right middle cerebral artery with a suture, threading it to a depth of 17–19 mm. In the sham operation group, the threading depth was approximately 10 mm.


We analyzed the expression of BDNF in different hippocampal regions by immunohistochemical staining of brain sections taken on post-operative days 7, 14, 21 and 30.


Sham operation group: We observed a number of a few BDNF-positive cells with light staining in the hippocampal CA1-CA4 regions and dentate gyrus. Cerebral ischemia group: compared with the sham operation group, BDNF increased on day 7, significantly increased on day 14, and reached a peak on day 21 (P < 0.05). Furthermore, immunologically reactive products were darkly stained, and neurons had long axons. BDNF was particularly highly expressed in the hippocampal CA3 and CA4 regions and dentate gyrus.


Cerebral ischemic injury can damage hippocampal neurons. Neurons can increase their anti-ischemic capacity by increasing BDNF expression in the hippocampal CA3 and CA4 regions and dentate gyrus.

Related Topics

    loading  Loading Related Articles