Tyrosine hydroxylase and phenylethanolamine-n-methyl transferase expression coexist in Purkinje cells of the rat cerebellum. Numerous reports have also been published addressing whether dopamine-beta-hydroxylase (DBH) expression exists in cerebellar Purkinje cells.OBJECTIVE:
To investigate the coexistence of DBH and activator protein-2α expression in rat cerebellar Purkinje cells.DESIGN, TIME AND SETTING:
A cell morphological study was performed at the Institute of Neuroscience, Chongqing Medical University, China in May 2007.MATERIALS:
Ten healthy Wistar rats, of either gender, aged 14 weeks, served as experimental animals. Rabbit anti-mouse DBH, goat anti-mouse activator protein-2α and rabbit anti-mouse β-actin (Santa Cruz Biotechnology, Inc., USA), horseradish peroxidase-labeled goat anti-rabbit IgG, FITC-labeled mouse anti-rabbit IgG, and Cy3-labeled mouse anti-goat IgG (Boster, Wuhan, China), were used in this study.METHODS:
Immunohistochemical staining was used to measure the expression of DBH or activator protein-2α, with double-label immunofluorescence being employed to determine coexpression of both, in the cerebellum of 5 randomly selected rats. Western blot assay was utilized to determine the expression of DBH and activator protein-2α in the cerebellum of the remaining 5 rats.MAIN OUTCOME MEASURES:
Expression, localization and coexistence of DBH and activator protein-2α in the cerebellum were measured separately.RESULTS:
Immunohistochemical staining demonstrated that cerebellar Purkinje cells stained positive for DBH and activator protein-2α. Western blot assay also demonstrated DBH and activator protein-2α expression in the cerebellum. Double-labeling immunofluorescence showed the coexistence of DBH and activator protein-2α in cerebellar Purkinje cells.CONCLUSION:
Norepinephrine and activator protein-2α coexist in rat cerebellar Purkinje cells.