To evaluate plasma levels of platelet auto-antibodies in patients with thrombocytopenia in pregnancy and assess the clinical significance.METHODS:
A total of 54 pregnant women with thrombocytopenia, 44 normal pregnant women and 28 women with immune thrombocytopenia (ITP), as well as 31 healthy women were enrolled in this study. The plasma levels of platelet auto-antibodies against 5 platelet glycoproteins (GPIX, GPIb, GPIIIa, GPIIb and P-selectin) were detected by flow cytometric immuno-beads array. The difference was analyzed statistically, and the receiver operating curve (ROC) was constructed to assess the diagnostic value.RESULTS:
Compared with normal pregnant women and healthy controls, the plasma levels of auto-antibodies against glycoprotein GPIX and GPIb were significantly increased in pregnant women with thrombocytopenia (P < .05), while the levels of other 3 auto-antibodies were similar. The positive rate of auto-antibodies in pregnancy women with thrombocytopenia was higher than that in normal pregnant women (59.3% vs 31.8%, P < .01). ROC analysis showed that the auto-antibody anti-GPIX had a high diagnostic value in pregnant women with thrombocytopenia over normal pregnant women with the area under the curve value of 0.779 (P < .001), the sensitivity and specificity were 76.3% and 100%, respectively.CONCLUSION:
The plasma profile of platelet auto-antibodies in patients with thrombocytopenia in pregnancy was different from that in ITP patients. The auto-antibody anti-GPIX may be served as a biomarker of the disease.