Culturally sensitive and valid assessments of participation in occupations are needed for Spanish-speaking children to enhance services offered to this population. The purpose of this study was to assess the validity and reliability of the newly developed Spanish version of the Children's Assessment of Participation and Enjoyment and Preferences for Activities of Children, the Evaluación de Participacion y Disfrute de los Niños y Preferencias de las Actividades de los Niños (EPDN/PAN). The sample included 249 children with and without disabilities, aged 6 to 15 years. Statistically significant differences (p < 0.05) in the participation of children with disabilities and children without disabilities, and between younger and older children were found. No statistical significant differences were found between males and females in the dimensions of participation assessed. Adequate internal consistency (0.70–0.92) was found for the PAN scale and the Overall and Informal domain scores of the EPDN-Intensity scale. Further study is needed to evaluate the formal domain scale and gender differences by activity types.