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Safety and health culture is about the perception shared by the organisation‘s members towards safety and the effort to maintain the safety and health level in the organisation. Our study concerned safety and health culture assessment in 600 companies of the private sector in Côte d’Ivoire. Preliminary results for 151 companies were presented in the present communication and concerned sectors of transport, manufacturing, construction and publics work, forest exploitation and sawmills.A descriptive cross-sectional study was conducted from November to December 2016 in 151 companies. But, since all companies having more 100 workers in Côte d’Ivoire are concerned, soapproximately 6600 workers will be includedat the end of our study. For the 151 companies, 1661 workers were questioned about their opinion on the implementation level in their companies of prevention culture pillars as confirmed at the 2nd Strategic Conference of Dresden (Germany, 2011) and leading role criteria as defined in 2012 by the Agency European for OSH. In addition, professional and sociodemographic characteristics of workers have been noted.Results, covering 151 companies, indicated that 70% of the workers (n=1661) affirmed effective implementation of the prevention culture in their companies. About the pillars of the prevention culture, 68% of workers affirmed occurrence of reducing work accident and occupational diseases scheme in their companies, 55%for the OHS training program and 51% for the program of cooperation between public health and OSH. According to the leading role, 51% of workers affirmed relatively high OSH prevention policy in their companies. However, in the manufacturing sector (n=58), prevention policy is inadequate in 44% of cases and completely non-existent in 23% of cases.Taken together, in contrast to the positive perception of workers, our results suggested an insufficient implementation of safety and health culture in the 151 companies.