Oxidative stress leads to many kinds of diseases. Various chemicals in workplaces induce oxidative stress. In addition, psychological stress also increases oxidative stress. To prevent diseases potentiated by oxidative stress, a method for the appropriate assessment of the oxidative stress status is needed. Currently, urinary 8-hydroxydeoxyguanosine (8-OHdG) is widely measured as an oxidative stress biomarker. There is a specific advantage if saliva can be used as the sample to measure the oxidative stress biomarker, because saliva is much easier to collect than urine. However, the accurate measurement of 8-OHdG in saliva is impractical, because the quantity of 8-OHdG in saliva is quite low. In this study, we investigated the measurement of 8-hydroxyguanine (8-OHGua) in saliva, as an oxidative stress marker.Methods
The 8-OHGua levels in saliva were analysed by a column switching HPLC system equipped with an electrochemical detector (HPLC-ECD).Results
The 8-OHGua in saliva could be detected as a single peak by HPLC-ECD. The salivary 8-OHGua levels of smokers were significantly higher than those of non-smokers.Conclusion
Salivary 8-OHGua may be a useful biomarker in the human population, in relation to the assessment of the oxidative stress induced by various factors in working environments.