657 Mineral oil is a risk for autoantibodies induction


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Abstract

IntroductionMineral oil(hydrocarbon)is also used in many factories, and workers are exposed to a lot kind of mineral oil. It was reported that one element mineral oil named pristane induced inflammatory arthritis in rats and also induced lupus-associated autoantibodies such as antissDNA, anti-Su and anti-nRNP. Therefore, we investigated other mineral oils and evaluated the mechanism of autoantibodies induction.MethodsFemale BALB/cJ (4 weeks old) were used. At 3 months of age, target mineral oil (pristine and other mineral oils) was injected intraperitoneal of each mouse. After 3 months later from injection, we sacrificed mice and extracted peritoneal cells and spleen cells from mice, and evaluated autoantibodies induction. And we also evaluate the expression of Tcell, macrophage cells and B cells surface receptors (Tcell:CD28, ICOS, CD40L, PD1. Macrophage cell and B cell: ICOS-L, CD40, PD-L1).ResultWe indicated autoantibodies (Anti–nuclear, anti–ssDNA, anti–Su and anti–nRNP antibody) in mouse injected pristane and other mineral oils.We detected CD3ζchain reduction in T cell of mineral oil injected mouse.Expression of all surface receptors (CD28, ICOS, CD40L, PD1) of T cell were increased.On the other hand, Macrophage cell and B cell surface receptors (ICOS-L, CD40, PD-L1) were decreased.DiscussionOur study indicated that mineral oil (pristane and other mineral oils) induced autoantibody, and CD3ζchain was reduced like to human autoimmune disease. Concerning to T cell, T cell receptors were stimulated but CD3ζchain was decreased. And macrophage cell and B cell were suppressed. Therefore, Mineral oil could stimulate T cell and suppress macrophage cell and B cell. But CD3ζof T cell was reduced. We think these phenomena could relate to induction of autoantibodies.

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