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There are various chemical agents such as organic solvents (OS), which can cause hearing loss. The objective of this study was to determine the presence of auditory dysfunction in a mixture of DO and noise-exposed workers from a printing press.Cross-sectional study was conducted including 176 from a printing press in Mexico City, exposed to noise and an OS mixturesWe categorised workers within 2 groups I. Exposed for <10 and II. Exposed ≥10 years, we estimated hearing loss through a multiple linear regression model.The mean age of group I was 32±9.3 [19–62] years and for group II was 41. 6±6. 5 [29–58] years. The mean noise was 78.10±10.6 dB58.1 dB and 93.8 dB, group I. showed a threshold fall in the 4 kHz up to 25 DB in both ears, with an average recovery of 5 dB at 8 kHz Right ear: 2000 Hz: II β=4.2 (p=0.003), 4000 Hz: II β=5.6 (p=0.002), 8000 Hz II β=3.8 (p=0.5); Left ear: 2000 Hz: II.=4.1 (p=0.002), 4000 Hz: II β=5.2 (p=0.006), 8000 Hz: II β=5.2 (p=0.002) the second model high frequencies (2, 4, and 8 KHz) in right ear was II β=4.4 (p=0.002) and in the left ear was II β=4.8 (p<0.001).Our studied population, showed an overall prevalence of auditory dysfunction of 3.94%, group II was the most affected Workers with a concomitant exposure to noise and DO >10 years have a higher auditory dysfunction prevalence, compared with workers without exposure to these agents.