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The current study examines the effectiveness of a newly developed work-family intervention program on one’s own work-to-family and family-to-work spillovers (both positive and negative) and work-family balance self-efficacy among Japanese dual-earner workers with pre-school child(ren).The study design was a single-group pre-post comparison. This intervention program was conducted as a pilot study of TWIN (Tokyo Work-life INterface) study III. Participants were eleven workers of a private company in Japan. They were all dual-earner workers with pre-school child(ren). The program consisted of seven 45 min sessions with a two-week interval between them. The outcomes were assessed by a questionnaire at baseline (Time 1) and post-intervention (Time 2). The paired t-test was conducted as an indicator of intervention effect. Effect sizes were calculated using Cohen’s d based on pre-post score differences (Trial registration: UMIN Clinical Trials Registry UMIN000025803).The program showed a medium and favourable intervention effect on work-to-family positive spillover (Cohen’s d=0.53), and small and favourable intervention effects on family-to-work positive spillover (Cohen’s d=0.33) and work-family balance self-efficacy (Cohen’s d=0.39). Unexpectedly, it showed a significantly small and adverse effect on work-to-family negative spillover (Cohen’s d=0.33).The newly developed work-family intervention program was found effective in improving work-family positive spillover (both work-to family and family-to work) and work-family balance self-efficacy among Japanese dual-earner workers with pre-school child(ren).