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This study was to understand the current situation of long working hours and identify the association of long working hours and job stress and depression among workers in state grid company.The project was done using the cross-sectional survey. All questionnaires were completed by self-administered with informal consent. Employees whose weekly working hours was 40 or less were set as the reference group, and the others were categorised with a gradient of 10 hours for grouping; the PHQ-9 scale used to assess depression, the job demand-control (JDC) Model used to evaluate job stress. And SPSS software to analyse data, multivariate Logistic analysis were performed to identify the association between long working hours and job stress, depression.Results there were 1069 staff attend this survey and 63.0% employee with standard working hour which they work for 40 hours a week. And 18.8% employees with 41–50 hours a week, and 12.9% workers with 51–60 hours a week, only 5.2% employees worked over 60 hours a week. There were 63.2% workers with job stress by job demand control ratio over 1.0% and 66.9% employee self-reported with depression by PHQ-9 over 5. The association between increasing weekly working hours and occupational stress and depression was statistically significant. Compared to the reference group, for those who worked more than 60 hours per week, the odds ratio of depression was 3.25 (95% CI: 1.43 to 7.40) times increased; the odds ratio of occupational stress was 4.34 (95% CI: 1.19 to 15.92) times increased; the odds ratio of moderate-severe and severe depressive symptoms was 11.18 (95% CI: 1.21 to 115.05) times increased.Long working hours can significantly increase the risk of job stress and depressive symptoms among the national grid company employees. When their weekly working hours exceeds 60, long working hours will be the independent risk factor for both job stress and depression.