Effects of enamel matrix derivative on gene expression of primary osteoblasts

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Objective.The aim of this study was to investigate the effects of enamel matrix derivative (EMD) on gene expression of collagen α1 (I), osteocalcin, prostaglandin G/H synthase 2 (PGHS-2), interleukin-6, and insulin-like growth factor I in primary mouse osteoblasts.Study Design.Primary osteoblasts were digested from 6- to 8-day-old mouse calvaria. Cells were divided into 4 groups and cultured for 24, 48, and 72 hours with a serum-free modified eagle medium as negative control, modified eagle medium with 25 μg/mL EMD, modified eagle medium with 100 μg/mL EMD, and modified eagle medium plus 10% fetal bovine serum as positive control. Gene expression was determined by Northern blot and reverse transcription polymerase chain reaction analysis.Result.EMD enhanced collagen I, interleukin-6, and PGHS-2 expression and did not stimulate the expression of osteocalcin and IGF-I.Conclusion.These results indicate that EMD might regulate certain gene expression during periodontal tissue regeneration.

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