Candida-associated denture stomatitis is a recurrent and debilitating oral mucosal disease. Development of anticandidal denture materials represents a promising strategy to manage this condition. We have previously shown that miconazole incorporated in methacrylic acid (MAA) copolymerized diurethane dimethacrylate (UDMA) denture materials has long-term anticandidal activity. In this study, we examined the ability of culture medium conditioned with drug-free- or miconazole-MAA-UDMA discs to preventCandidainfection in anin vitrooral epithelial cell/Candida albicanscoculture system.MATERIALS AND METHODS:
Candida albicans(C. albicans)-induced OKF6/TERT-2 cell damage was quantified by the release of lactate dehydrogenase from epithelial cells, cytokine production was quantified using protein cytokine arrays, and the expression ofC. albicansgenes was measured by RT-qPCR.RESULTS:
Candida albicanshad limited growth with altered expression levels of secreted aspartyl proteinase-2 and -5 in culture medium conditioned by miconazole-MAA-UDMA discs. Significantly, the ability ofC. albicansto induce oral epithelial cell damage and trigger epithelial proinflammatory cytokine production was also inhibited by miconazole disc conditioned media.CONCLUSION:
Miconazole released from MAA-UDMA denture materials effectively prevents the development of candidal infection in anin vitrooral epithelial system. Further characterization of this drug-rechargeable denture material is warranted.