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Ag(I) and Cu(II) complexes with 2-(4, 6-di-tert-butyl-2, 3-dihydroxyphenylsulfanyl)acetic acid ligands (L) have been synthesized and characterized using a combination of methods, including x-ray powder diffraction, elemental analysis, TG/DTA, FT-IR, and EPR. The antimicrobial properties of 2-(4, 6-di-tert-butyl-2, 3-dihydroxyphenylsulfanyl]acetic acid and its complexes with Ag(I) and Cu(II) were tested against standard E. coli, Ps. aeruginosa, C. albicans, B. subtilis, St. aureus, and Pr. vulgaris strains.