Immobilization of Penicillium solitum lipase on the carbon fiber material “Dnepr-MN”

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Abstract

A method for immobilizing Penicillium solitum lipase (the medicinal agent Solizyme) on the activated carbon fiber material “Dnepr-MN” is proposed; using a matrix with total pore volumes of 0.41–1.3 cm3/g, the method allows the enzyme:matrix ratio (1:30–1:60) to be controlled, yielding preparations with a specified lipolytic activity (20–40 LU/mg of agent) at quantitative yield (91–97%). Studies of the pH and temperature stability, functioning in acid media, and desorption demonstrated stabilization and prolongation of the lipolytic activity of the immobilized lipase.

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