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To examine predictors of blood glucose (BG) estimation accuracy and errors in adolescents with Type 1 diabetes.Seventy-eight adolescents (ages 11-19) rated their physical symptoms and estimated their BG prior to conducting daily BG tests. BG estimation data were subject to an error grid analysis. Hierarchical regression was used to identify predictors of BG estimation accuracy and errors.The average participant made accurate BG estimations 37% of the time and clinically relevant BG estimation errors 24% of the time. Girls and older adolescents had higher BG estimation accuracy rates and lower BG estimation error rates than boys and younger adolescents. Higher BG variability was also associated with increased BG estimation errors.