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Accurate temperature assessment in pediatric practice is of critical importance when diagnosing, treating, and monitoring illness. No official standardized clinical research identifies a thermometry measurement method meeting all practice needs for all combined pediatric ages and practice settings. We conducted an IRB-approved research study in 140 Pediatric Unit patients and examined the Exergen Temporal ScannerTM (TAT)-5000 compared to the current practices of axillary thermometry for newborn infants (ages 0 to 3 months), and tympanic thermometry for children and adolescents (3 months to 21 years old). Patient and room temperatures, demographic data, and clinical data were collected. Paired sample t tests and Bland-Altman analyses were used to examine thermometry differences and define statistical equivalence between thermometry methods. Mean axillary (98.06°F) and TAT (98.92°F) temperatures in newborns were significantly different [t (52) = -9.33, p<0.001], as were mean tympanic (98.06°F) and TAT (99.31°F) child-adolescent temperatures [t (72) = -11.28, p<0.001]. TAT was least impacted by ambient air temperature and statistically comparable to axillary thermometry, but not tympanic thermometry. TAT would be an adequate thermometry alternative for general pediatrics inpatients for body temperature monitoring in conditions such as asthma, hyperbilirubinemia, pneumonia, tonsillectomy, and post-operative appendectomy.