Utilization of Liposomes for Correction of the Metabolic and Bactericidal Deficiencies in Chronic Granulomatous Disease

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Abstract

Summary

IgG-coated liposomes containing glucose oxidase (GO) in order to provide a means of generating H2O2 were prepared. Leukocytes from patients with chronic granulomatous disease (CGD) which are lacking a means of generating H2O2, ingested the IgG-coated liposomes and metabolic oxidative deficiencies of glucose-1-14C oxidation and iodination were normalized. Both of these activities have been shown to depend upon the availability of H2O2 within polymorphonuclear leukocytes. Improvement in the capacity to kill Staphylococcus aureus by chronic granulomatous disease leukocytes were observed under similar conditions. Thus, it is possible to restore the oxidative metabolic capacities to CGD leukocytes by the introduction of glucose oxidase containing liposomes to these cells.

Speculation

It is possible to restore the oxidative metabolic capacities and to obtain moderate improvement in the bactericidal functions in vitro of CGD leukocytes by providing the cells ingestible liposomes containing GO. Further studies might define more efficient methods for introducing oxidase activity into these cells, or employing drugs that generate H2O2.

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