This study was designed to investigate the attitudes of university students who are prospective physical education teachers toward individuals with physical disabilities. 47 college students majoring in physical education (Study group) and 34 students from other sports-related departments, i.e., sports management and training (Control group), participated in the study. The study group took a 14-week course on Adapted Physical Education (APE), whereas the control group did not. The Attitude Toward Disabled Persons scale, Form O was used to compare the attitudes of the groups. Analyses showed no significant difference between the groups in terms of attitude towards individuals with physical disabilities at the beginning of the semester but a significant difference at semester end. Results indicated that the APE course positively influenced attitude of the prospective physical education teachers towards individuals with physical disabilities.