In a 2006 study the influence of spacing between vertical lines, exposure time, and number of vertical lines on visual performance was identified. The present study further explored the effects of luminance ratio on discrimination of separation. Analysis showed the luminance ratio of target vertical lines affected the visual performance of both younger and elderly people. The luminance ratio was greater, as was accuracy; however, accuracy might not become greater when the luminance ratio was too high. Further, the effect of the number of vertical lines on accuracy was significant, as was consistent with the 2006 study. Two interactive two-way effects were found, group × luminance ratio and luminance ratio × number of vertical lines.