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Cloning of the sulfakinin transcript reveals two sulfakinins in Rhodnius prolixus..Rhopr-SK transcript expression is present in neurons in the brain.Rhopr-SK-1 induces contractions of the hindgut in a dose-dependent manner.Rhopr-SK-1 injection decreases the weight of the blood meal consumed.Sulfakinins (SKs) are a family of multifunctional neuropeptides that have been shown to have myotropic activity on muscles of the digestive system and to function as feeding satiety factors. Here, we confirm via cloning the presence of two sulfakinins (Rhopr-SK-1 and Rhopr-SK-2) in Rhodnius prolixus. Reverse transcriptase quantitative PCR demonstrates that the Rhopr-SK transcript is highly expressed in the central nervous system (CNS) of unfed fifth-instar R. prolixus. Fluorescent in situ hybridization shows transcript expression only in neurons in the brain. Immunohistochemical staining of SK-like peptides was observed in the same neurons in the brain and in processes extending throughout the CNS, as well as over the posterior midgut and anterior hindgut. Rhopr-SK-1 (sulfated form) induces contractions of the hindgut in a dose-dependent manner. Injection Rhopr-SK-1 (sulfated form) significantly decreases the overall weight of the blood meal consumed, suggesting SK's role as a satiety factor in R. prolixus.