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We present a combined Sr, Nd, Pb and Os isotope study of lavas and associated genetically related megacrysts from the Biu and Jos Plateaux, northern Cameroon Volcanic Line (CVL). Comparison of lavas and megacrysts allows us to distinguish between two contamination paths of the primary magmas. The first is characterized by both increasing 206Pb/204Pb (19·82–20·33) and 87Sr/86Sr (0·70290–0·70310), and decreasing εNd (7·0–6·0), and involves addition of an enriched sub-continental lithospheric mantle-derived melt. The second contamination path is characterized by decreasing 206Pb/204Pb (19·82–19·03), but also increasing 87Sr/86Sr (0·70290–0·70359), increasing 187Os/188Os (∼0·130–0·245) and decreasing εNd (7·0–4·6), and involves addition of up to 8% bulk continental crust. Isotopic systematics of some lavas from the oceanic sector of the CVL also imply the involvement of a continental crustal component. Assuming that the line as a whole shares a common source, we propose that the continental signature seen in the oceanic sector of the CVL is caused by shallow contamination, either by continent-derived sediments or by rafted crustal blocks that became trapped in the oceanic lithosphere during continental breakup in the Mesozoic.