Deciphering O-glycomics for the development and production of biopharmaceuticals

    loading  Checking for direct PDF access through Ovid


The functional impact of glycosylation on drug efficacy and safety profiles has been demonstrated in a wide range of biopharmaceuticals. Understanding of the N-glycosylation pathway and the advent of analytical technologies has enabled both detailed and rapid characterization of N-glycans, thus providing critical insights for regulatory compliance. In comparison, O-glycosylation encompasses several types of protein modifications by a more heterogeneous pool of sugars, and the understanding of its biology is less mature. Concomitantly, there exist several limitations in O-glycan analytical strategies, impacting both O-glycoprotein-based drug development and regulatory compliance. Here, we aim to provide a critical review of the biology and functional importance of O-glycosylation in the context of existing and potential O-glycoprotein drugs, as well as the corresponding analytical methods. The ultimate goal is to identify the gaps in current analytical methods and propose potential future directions for O-glycosylation analysis, to support the development and production of O-glycosylated biopharmaceuticals.

    loading  Loading Related Articles