The present study was conducted to study the effect of 24-epibrassinolide (EBL) on changes of plant growth, net photosynthetic rate, carbonic anhydrase (E.C. 184.108.40.206) and nitrate reductase (E.C.220.127.116.11) activities in the leaves of Raphanus sativus L. under the influence of cadmium (Cd) stress. Cd reduced plant growth, photosynthetic pigment levels, net photosynthetic rate and the activities of carbonic anhydrase and nitrate reductase. However seed application of EBL reduced the toxic effect of Cd on plant growth, pigment content, photosynthesis and enzyme activities. The studies clearly demonstrated the ameliorating effect of 24-epibrassinolide in mitigating the toxicity of Cd in plants.